回到网站

The QIF method was initially tailored to the determination of serum antibodies to Toxoplasma gondii by measuring the fluorescence intensity of individual tagged organisms

 The nonspecific fluorescence intensity resulting from the counterstain was eliminated by use of a red-suppressing filter. The dilution-correlated polar fluorescence component was removed by subtraction of the intensity for the matching negative control Barauskene LP.. BABASKIN IM.. Schouenborg L, Fischer A, Schrøder SS.. A control system for monitoring the performance of leukocyte strip tests.. Pendergrass JH, Skjold AC, Cattell JA, Stover LR.. The control system described here is used to monitor the performance of urinary reagent-strip tests for leukocytes.. The presence of leukocyte esterase in the urine is used as a marker for leukocytes in urine.. The control system is based on sonicated leukocytes, isolated from whole blood.. The esterase activity of this sonicate is determined by spectrophotometry with the N-tosyl-L-alanine determine the amount of sonicate needed to prepare buffered esterase-containing at room temperature.. The variability of the control system was tested by preparing it five times in a day on five separate days.. The overall CV for Leukostix Reagent Strips (Ames Division, Miles Laboratories, Inc..) when tested Kutter D.. von Studnitz W.. Pitkänen E.. Berg B, Brönnestam R, Erlanson P, Fjellström, Fritz H, Hellsing K, Jagenburg R, Tibbling G.. van Eijk HG.. Vögtle-Junkert U.. Microstix test strips are a new aid in the diagnosis of yeasts which are easy to handle and can be used universally.. Observations on clinical material (a total culture.. The test strips are not absolutely yeastspecific, since problem organisms and molds may cause a brown discoloration.. False negative findings are occasionally simulated by C.. tropicalis and C.. mycoderma.. Vonderschmitt D, Scholer A.. Commercial test strips using the protein error of certain indicators for the colorimetric detection of protein (especially albumin) were compared with a quantitative nephelometric method.. The study proved that there is good agreement between the results from the quantitative method and those from the test strips separation of the segments for semi-quantitative determinations.. There was a very good agreement between the test strips in protein-containing urines.. In proportion of false-positive results.. Berger W.. Kuse R, Hausmann K.. By a simple program of quality control for the Coulter counter model S two deficient reagents could be detected.. To high values of PCV associated with a decrease of MCHC were caused by a faulty Isoton batch.. This error was revealed leukocyte counts due to insufficiently lysed giant platelets and platelet aggregates were produced by modified Lyse-S batches.. Comparisons made with a Coulter counter model F using Zaponin and with a counting chamber yielded values at lower levels.. Kragh PL, Madsen HG.. Slama G, Hautecouverture M, Assan R, Eschwege E, Tchobroutsky G.. Chen IJ, Fang YJ, Cheng JJ, Hsu HY.. Effects of surfactants or aprotinin on antithrombin III assay.. Gram J, Jespersen J.. Commercial dry-reagent tests as a route for staff contamination.. Grant J, Ahmed SA, Young A, Young SE.. A new colorimetric reagent for micro determination of ammonia.. STONE WE.. Attempts at standardization of a highly sensitive Schiff reagent.. Lodin Z, Faltin J, Sharma K.. Indefinite preservation of leucofuchsin in the P..A..S.. Schiff method.. SIMARD R, D'ANGELO G.. Gutensohn G, Boesken W, Weisshaar D, Hellsing K, Tritschler W, Banauch D, Besenfelder E.. Spontaneous rupture of membranes.. Rapid strip tests for detection.. Steinman G, Kleiner GJ, Greston WM.. Clinical trial of leukocyte test strips in routine use.. Gelbart SM, Chen WT, Reid R.. Home monitoring of blood glucose using filter paper strips.. Paisey R, Bradshaw P, Hartog M, West P.. COULTER lyse S IIIdiff: reagent lot gives erroneous results.. Kattermann R, Naroska I.. Bonard C, Darioli R, Petrillo A, Cretin T, Marazzi A.. hematuria-detecting dipsticks are fully reliable.. Dipsticks therefore render superfluous microscopic examination of normal samples, which represents more than half of the analyses performed in ambulatory care.. Kutter D, van Oudheusden AP, Hilvers AG, Nechvile K, van Buul T, Koller PU.. Comment in Rudelic I.. Autenshlius AI, Isengel'dina BB, Kazenas EL.. GEYER G.. Efimov GI, Libina AA, Megrelishvili AG, Finogenov AD, Shvetsova VA.. A special unit is developed to operate in the semi-automatic biochemical set.. It provides mixing and thermostatic control of a reagent mixture necessary for biochemical studies.. To hold the thermostatic liquid at the preset temperature capacity of the thermostat.. Tschudi P.. This report is an assessment of clinical chemistry dry-reagent methodology for veterinary use.. A portable reflectance photometer and dry-reagent strips were used to measure total bilirubin, glucose, creatinine, creatine-kinase and urea in equine, bovine and canine sera.. peroxidase uses following aspects of performance are reported: within run variation determined on animal samples, between run variation using a commercial control serum, correlation between dry-reagent and wet-reagent methodology on clinical samples.. Results of the above analytes have shown a satisfactory precision and indicate an excellent correlation with the reference method of our laboratory.. Minimal technical requirements, simplicity, reproducibility and accuracy comparable with wet-reagent methods are the technical advantages of this system.. Momose T, Okura Y, Imaizumi T.. Janda J, Eliásek J, Sikut M, Kotalová R.. Holzer H, Pogglitsch H, Katschnig H.. Podoplekina LE, Laptakova LM, Shutova NA, Unger GN, Moriakin AV, Sharova OI.. Midro A.. Production of an anticanine globulin (polyspecific) reagent for laboratory investigation.. University, Chiang Mai, Thailand.. OBJECTIVES: Canine allo- or autoantibodies are clinically important, but attachment of these immunoglobulin G (IgG) antibodies does not produce observable haemagglutination.. Antibody to canine globulins is required to demonstrate sensitisation of red blood cells.. Commercial reagents are available, but these often differ in sensitivity and specificity.. Rabbit anticanine globulins (polyspecific) were produced for use in canine blood compatibility testing and in the investigation of immune-mediated haemolytic anaemia.. METHODS: Canine sera was pooled, IgG was purified and subsequently used to immunise rabbits.. A rising titre of anticanine IgG was demonstrated by indirect enzyme-linked immunosorbent assay.. Rabbit anticanine complement was isolated and investigated by agglutination of complement-coated canine red blood cells.. Both antibodies were purified and checked for crossreactivity before being combined to polyspecific anticanine globulins.. The obtained reagent was used to indicate sensitised canine red blood cells and free antibodies in three different types of clinical samples, including blood for compatibility testing and that for investigation of immune-mediated haemolytic anaemia and screening for post-transfusion alloantibodies and was also compared to commercial Coombs' reagent.. RESULTS: The product provided results in accordance with those from commercial CLINICAL SIGNIFICANCE: This product is helpful for canine crossmatching purposes and in the investigation of immune-mediated haemolytic anaemia.. group IIA cations.. reagent for the qualitative identification of alkali metal (Group IA) and alkaline earth (Group IIA) cations.. This methodology was based on the characterization of observed crystal morphologies, since a unique crystal habit could be associated with each adduct product.. To understand the scientific foundations which permitted chemical microscopy to function as a useful analytical technique, the products formed between dilituric acid and the Group IA and IIA cations were characterized using polarizing optical microscopy, powder X-ray diffraction, thermal analysis and solid-state nuclear magnetic resonance.. It was found that the origins of the different crystal morphologies associated with each of the adduct arose from the ability of the systems to form various hydrate species, which could also contain structural variations due to cation/diliturate packing patterns.. Cystic fibrosis detection by means of a test-strip.. where to buy peroxidase , Busch EW, Kollberg H, Hellsing K..

peroxidase uses|where to buy peroxidase